Agilent's ChemStation and Ion Signature Technology's Ion Signature
quantitative deconvolution software were compared for statistical differences in
accuracy. A mixture of PAHs, Pesticides, and PCBs was prepared. This
standard solution was spiked with a gasoline/engine oil mixture and
then analyzed using EPA SW 846 Method 8270C by GCMS. (PCBs and Pesticides
are usually analyzed by GC/ECD.) The data shows the percent recoveries
for the 40-minute Agilent-analyzed sample and the 5-minute Ion Signature results.
Note that no sample cleanup
was used for the Ion Signature 5-minute analysis. |
Result: Both sets of results
are statistically the same. and both meet EPA data quality requirements(with
one exception - see highlighted result). The Ion Signature deconvolution
algorithms positively and acccurately identified each compound in
a highly complex matrix with no sample cleanup -
but only needed a 5-minute run |
The EPA's conclusion after using this software: “…the
IFD (Ion Signature) deconvolution algorithms are capable of identifying low
concentrations of target analytes in the presence of high levels
of matrix interferences. By "seeing through" the matrix
interferences, the software reduces the need for sample re-analysis
and dilution, and increases confidence in surrogate, internal standard,
and target compound identification and quantification.”
(http://www.clu-in.org/download/char/hafbcs2.pdf)
|
| Method |
EPA SW 846 Method 8270 C |
| Software |
Agilent Chemstation® |
Ion Signature® |
| Sample Clean Up |
Yes, SPE |
None |
| Run Time |
40 |
5 |
| |
|
|
| Targeted Compounds |
% Recovery of Standard / Oil Mixture |
| Acenaphthene |
112 |
106 |
| Acenaphthylene |
109 |
96 |
| Aldrine |
120 |
89 |
| Alpha-BHC |
92 |
103 |
| Benzo(a)pyrene |
120 |
118 |
| Benzo(g,h,i)perylene |
91 |
91 |
| Benzo[a]anthracene/Chrysene |
96 |
96 |
| Benzo(b)/(k)Fluoranthene |
116 |
113 |
| Beta-BHC |
95 |
95 |
| Cl-2 |
120 |
102 |
| Cl-3 |
96 |
96 |
| Cl-4 |
111 |
106 |
| Cl-5 |
96 |
92 |
| Cl-6 |
128 |
87 |
| Cl-7 |
96 |
93 |
| Chlordane |
111 |
109 |
| DDD |
103 |
103 |
| DDE |
97 |
100 |
| DDT |
99 |
100 |
| Delta-BHC |
83 |
93 |
| Dibenz(a,h)anthracene |
83 |
87 |
| Dieldrine |
124 |
119 |
| Endirn aldehyde |
86 |
86 |
| Endosulfan 1 |
126 |
123 |
| Endosulfan 2 |
123 |
123 |
| Endosulfan sulfate |
131 |
122 |
| Endrin |
124 |
101 |
| Endrin keton |
89 |
84 |
| Fluoranthene |
110 |
110 |
| Fluorene |
95 |
95 |
| Gamma-BHC |
88 |
85 |
| Heptachlor |
118 |
118 |
| Heptaclor Epoxide |
112 |
117 |
| Indeno(1,2,3-c,d)pyrene |
88 |
90 |
| Methoxychlor |
122 |
122 |
| Naphthalene |
148 |
128 |
| Phenanthrene/Anthracend |
92 |
96 |
| Pyrene |
116 |
116 |
| Average (%
RSD) |
107 (15 %) |
103 ( 12%) |
EPA, Innovations in Site Characterization, Office of Solid Waste and Emergency
Response, EPA-542-R-98-006. "Ion Signature Technology reduces the
need for re-analysis and dilution, and increases confidence in surrogate,
internal standard, and target compound identification and quantification."
|
